Tool list

bwc bench with cellink

General Lab Equipment

  • Fumehoods
  • Bench space for experimental set up
  • Scales and balances (micro and gross)
  • Sonicating water bath
  • Probe tip sonicator
  • Micropipets, serological pipets, and multi channel pipets (both standard and electronic)
  • Microfuges 
  • Sonicators:
    • Probe tip
    • Water Bath 
  • Lyophilizer
    • Cabinet and Jar style
  • pH meter(s)
  • Microwave
  • Hybridization oven
  • Heat/Stir plates
  • All standard laboratory glassware
  • Flammables storage
  • Distilled water

Tissue Culture and Human Samples

  • Dedicated Biosafety Level 2 (BSL-2) and Human Pathogens & Toxins Act (HPTA) certified space
  • Class IIA Biological Safety Cabinet
  • CytoFLEX Flow Cytometer (Beckman Coulter)
    • 3 lasers, 6 colours, 8 parameters, 13 dye band-pass filters
    • Violet 405nm , Blue 488nm, Red 638nm with FSC and SSC.
    • 96 well plate loader module with mode control switch
  • Bright field and Fluorescence Imager (Cytation 5, Biotek)
    • Live cell imaging with CO2 and incubator functions
    • 25x apo, 4x, 20x, 60x objectives
    • Adaptors that can image T-25 plates, Petrie dishes, 96,48, 24, and 12 well plates, and slides
    • DAPI, GFP, Texas Red
  • 3D Tissue Printer (Celllink)
  • CO2 Incubators
  • Pipette controllers for serological, plastic, single-use, pipets
  • Dedicated micropipets
  • Water bath
  • Frosty (for preparing cryo storage of cell lines)
  • Automatic cell counter (Countess, Invitrogen)
  • Inverted Tissue Culture Microscope (10x – 40x)
  • Water bath
  • Cold and Cryogenic Storage
    • Fridge, -20°C, -80 °C, LN2

Bacteria and Pathogens

  • Dedicated Biosafety Level 2 (BSL-2) and Human Pathogens & Toxins Act (HPTA) certified space
  • Class IIA Biological Safety Cabinet
  • Multiple temperature controlled Incubators
    • Shaking, non-shaking, variable temperatures, up to 20 L at a time
  • Optical Density Meter (OD 600 for Bacterial culture)
  • Bacterial culture glassware
  • Culture tubes, range of baffled flasks from 125 ml to 2000 ml
  • Cold and Cryogenic Storage
    • Fridge, -20°C, -80 °C, LN2
  • Bench top Autoclave

Nucleic Acids

  • Polymerase Chain Reaction Thermocyclers
    • Standard thermocycler
    • Quantitative (qPCR) QIAquant 5 plex (Qiagen)
    • Digital (dPCR) QUIAcuity One 5 plex (Qiagen)
  • PowerLyzer Tissue disruptor/bead mill (24 sample)
  • RNA-Only Room
    • Separate, authorized-use only access
    • Dedicated RNA-Only use equipment with gel running and extraction equipment
  • Magnet stands for bead pull downs
  • Vortex Mixers
    • Standard multi-speed (tubes, plates, and flat top heads)
    • Mini (Eppendorf tubes)
  • Centrifuges
    • Room temp and refrigerated
    • Plates
    • Eppendorf tubes, Falcon tubes, 50 ml, 500 ml, 1000 ml
    • Low speed and high speed
    • Sorvall Lynx (68,905 x g and up to 4L capacity, with refrigeration)
  • Agarose Gel casting trays and running apparatus
  • Agarose Gel imager
  • Stock solutions for Gels
  • 2ul Nucleic acid quantification spectrophotometer (8 samples +2 blanks at a time)
  • Micropipettes – Manual, Single Channel and Multi-channel
    • P1, 2, 10, 20, 200, and 1000
  • Electronic Micropipettes – Programmable Single Channel and Multi-channel
  • Micropipette Tips (Sterile & Non-Sterile)
  • Eppendorf tubes (Sterile & Non-Sterile)
  • Pipettes – sterile
    • 5 , 10, 25 mL

Peptides and Proteins

  • Peptide synthesizer (MultiPep-1, CEM)
    • 8-column, 96-well, and SPOT array modules
  • Cloning infrastructure
  • Bacterial Expression infrastructure
  • Probe tip sonicator for cell lysis
  • Large scale refrigerated centrifuge (4x 1L and 8x 50 ml up to 68,905 x g)
  • Large scale shaking-heating-cooling incubators with glassware
  • Polyacrylamide gel casting and running apparatus (up to 4 gels at a time)
  • Gel Staining trays and drying frames
  • Western Blot apparatus
  • Western Blot stock buffer solutions
  • Dialysis equipment
  • 2ul protein quantification spectrophotometer (8 samples +2 blanks at a time)

Biophysical Analysis and Spectroscopy

  • Fluorescence Imaging Plate Reader that can quantify fluorescent and absorbance output on imaging samples (Cytation 5, Biotek)
  • 2 Multimode Plate Readers (Cytation 5, Biotek and SpectraMax M5, Molecular Devices)
    • 96 well plates, 384 well plates , cuvettes, and 2 ul samples
    • Full spectral scan
    • UV-Vis absorbance
    • Fluorescence Polarization
    • FRET & TR-FRET
    • FLINT
    • Kinetic, endpoint, scanning
      • including excitation scans with fixed emission and fixed emission with excitation scan
    • Robotics liquid handler for in-instrument dispensing into plates (for setting up calibration curves or checkerboards and for reaction monitoring)
  • Fluorescence Imaging Plate Reader that can quantify fluorescent and absorbance output on imaging samples
  • Surface Plasmon Resonance Biacore X100 (GE)
  • Biolayer Interferometry (Blitz, Sartorius) for measuring binding kinetics